重叠PCR
重叠PCR
方法用PCR方法扩增il_3信号肽序列和人内皮抑素基因,然后采用重叠延伸pcr拼接的方法将两者拼接和扩增。
Conclusion Splicing overlap extension by PCR is a convenient method to obtain recombinant gene.结论:重叠延伸pcr是一种获得重构基因的简捷方法。
The common overlap extension PCR was improved and the new methods named as one-step overlap extension PCR, which can reduce the PCR times and improve the efficiency.对常规重叠延伸P CR技术进行了改进,改进后的方法称之为一步法重叠延伸pcr,减少了进型PCR反应的次数,提高了效率。